JOHN McMahon has criticised the behaviour of Tranmere's fans who greeted Ash Taylor's arrival on the pitch last Saturday with booing as "poor and disappointing."
Assistant manager McMahon is urging Rovers supporters to play a more positive role in backing their team.
Taylor was left out of the side for the goalless draw with Stevenage at Prenton Park as veteran Ian Goodison was paired with a loan signing Adam Dugdale at the heart of the defence.
Taylor joined the action as a 59th minute substitute when Goodison went off with a hamstring strain and was greeted, McMahon said, with booing from a large number of fans in a crowd of 4156.
McMahon said: "I thought it was really poor for a young player of ours to get that kind of reception when he came on.
"It was fine to cheer Goodison when he's coming off but for the majority of fans to boo their own player when he comes on to the pitch is poor from the crowd. It can't be good for team morale and it can't be good for the player."
McMahon added: "To be fair to Ash he did well when he came on. He showed a good attitude, strength and character.
"He has been getting criticism from certain elements of the crowd this season.
“Everyone says fans are entitled to voice their opinion because they pay their money. I will take that on board but what I can't take is the way people jump on the back of what others are doing and it becomes a big thing. It is wrong and it is disappointing. He is our player."
Taylor, 23, from Eastham, Wirral, is one of Tranmere's own.
He progressed through the youth development set-up and has made 166 senior appearances since 2009.
Last season he formed a defensive partnership with loan signing Ben Gibson, providing the defensive backbone of a Rovers team that led the division into January and conceded just 48 goals in the 46 league game programme.
This season Taylor found it difficult to develop an understanding with summer signing Stephen Foster, whose contract was cancelled last month after the 32-year-old struggled with his form and mobility.
McMahon said: "Ash did well for us last season. He's had a bit of a sticky patch but that is no reason for fans to boo their own player.
"They have to stick behind everyone. We have a squad of players here but the fans are part of the team too. We should all be on the same side, everyone together. Showing unity and strength comes from the spectators as well."
McMahon said he had been encouraged by Tranmere's performance against Stevenage, particularly in the defensive areas.
"We were a lot more solid at the back," he said. "We did not look as vulnerable when we were being attacked. The clean sheet will instil confidence in the defenders and the team as a whole."
McMahon reported the injury that forced Goodison to come off is "not too serious" and the Jamaican should be available for this Saturday's League One encounter with Brentford at Prenton Park.
l Tranmere spent £10,000 on agents' fees last season, one of the lowest figures in League One. Only Crewe Alexandra and Hartlepool United, who paid nothing to agents, and Walsall, who handed over £3,000, spent less.
Promoted Bournemouth handed over £688,666 in fees to agents, Coventry City spent £252,256 and Preston North End £199,605.